Composite

Part:BBa_K4171016:Design

Designed by: HUANG, YEN-JUNG   Group: iGEM22_NCKU_Tainan   (2022-08-16)


PT7-tRNA(UTuX)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 61


Design Notes

None


Source

1. T7 promoter sequence derives from the T7 bacteriophage

2. tRNAUTuX sequence is a variant of Escherichia coli tRNAsec.

References

Fu, X., Crnković, A., Sevostyanova, A. and Söll, D., 2018. Designing seryl‐ tRNA synthetase for improved serylation of selenocysteine tRNA s. FEBS Letters, 592(22), pp.3759-3768.